NAME                                                

                                                                        LabDay                                                           

 

EXERCISE 1

 

BIOLOGICAL MEASUREMENTS

 

 

INTRODUCTION

 

     Thescientific community utilizes a common basis for measuring, calculating, andexpressing numerical values and experimental data. This laboratory exerciseexplores the use of scientific notation and the treatment and analysis of data.

 

SCIENTIFIC NOTATION - EXPONENTS

 

     Mostnumbers we encounter on a daily basis can be written as whole numbers orfractions. This method is easy for small values but can become cumbersome forlarge numbers and very small fractions. Expressing a number in its exponentialfashion can simplify and help differentiate awkward numbers. The process ofchanging a number to its exponential form is to have a single digit to the leftof the decimal point with fractions and exponents to the right of the decimalpoint. See Table 1-1. Exponents can be either positive for numbers greater thanone or negative for numbers less than one. A higher exponent represents alarger number while a more negative exponent represents a smaller number.

Table1-1. Exponents.

 

Common Number

 

 

 

Exponential Number

4000

=

4.0 times 1000

=

4.0 x 10 3

750,000

=

7.5 times 100,000

=

7.5 x 10 5

0.0003

=

3.0 times 1/10,000

=

3.0 x 10 -4

0.0000864

=

8.64 times 1/100,000

=

8.64 x 10 -5

 

     Convertthe numbers on the left in Table 1-2 to exponential numbers on the right.

 

 

 


Table 1-2. Exponent Calculations.

 

Number

Exponential Number

60,000

 

570,000

 

3,630,000

 

0.00003

 

0.0000056

 

0.0000000044

 

 

 

     Arrange thefollowing numbers from the smallest to the largest by using their letters.


 

                      A        2.3x 10 -3                                             

                      B        1.8x 10 - 5                                             

                      C        5.0x 10 4                                              

                      D        4.6x 10 4                                              

                      E        2.0x 10 7                                               

                      F        2.0x 10 -2                                             

 

 

SCIENTIC NOTATION -METRIC SYSTEM

 

     Scientists,clinicians and most countries utilize the metric system as a standardizingsystem for measuring the physical and biological world around them. The metricsystem is based upon powers of ten where units of measurement increase ordecrease by tens, hundreds or thousands, etc. The standard unit of length isthe meter, mass (weight) the gram and volume the liter. Various prefixes areused with the metric system to represent units of measurement larger or smallerthan the standard unit and are applicable to length, mass and volume. The mostcommon prefix for values larger than the standard unit is kilo- whichrepresents a thousand standard units. The more common prefixes used in biologywhich represent fractions of the standard unit are deci-, centi-, milli-,micro-, and nano-. See Table 1-3. There are one thousand nano- units in amicro- unit and one thousand micro- units in a milli- unit. One thousand milli-units are in the standard unit of measurement. Thus 1000 times 1000 equals onemillion nano- units for each milli- unit. There are also one billion nano-units per standard unit, 1000 times 1000 times 1000.

 

 


Table 1.3. Metric System prefixes.

 

Prefix

Symbol

Value Compared to the Standard Unit

kilo

k

1.0 x 10 3

deci

d

1.0 x 10 -1

centi

c

1.0 x 10 -2

milli

m

1.0 x 10 -3

micro

µ (mu)

1.0 x 10 -6

nano

n

1.0 x 10 -9

 

 

LENGTH

     Thestandard unit of length is the meter (m) and is equivalent to 39.37 inches orjust over one yard. The common metric lengths used in biology include thecentimeter (one hundredth of a meter), the millimeter (one thousandth of ameter) and the micrometer (one millionth of a meter). Most cells range from 50to 200 micrometers in diameter.
See Figure 1-1.

Figure 1-1. Length relationships frommeters to micrometers.

 

MASS OR WEIGHT

     Thestandard unit of mass is the gram (the amount of artificial sweetener in thesugar packages found in family restaurants). The common weights seen in biologyrange from large kilogram organisms to small nanograms of chemicals, which arelocated within the body's fluid. See Figure 1-2.

 

                                 

            one gram        =                                1,000 mg       =           1,000,000 µg   =      1,000,000,000 ng

 

Figure 1-2. Mass Relationships fromGrams to Nanograms.

VOLUME

 One liter = 1000 ml =1,000,000 µl

     Thestandard unit of volume is the liter and is equivalent to 1.06 quarts. Volumein the metric system can be expressed in liters or in cubic measurements oflength. The common units used in physiology are the milliliter (one thousandthof a liter) and the microliter (one millionth of a liter). One milliliter isalso equal to a cubic centimeter (cc or cm3) and a microliter is equivalent to acubic millimeter (mm3).Figure 1-3.

 

 

 

Figure 1-3. Liter Diagram.

 

 

     Reviewthe material in Table 1-3, Figure 1-1, Figure 1-2, and Figure 1-3 and calculatethe following conversions.

 

1.     How manymilliliters are in one liter?

                                                                                                                  

 

2.     How manymicroliters are in one milliliter?

                                                                                                                  

 

3.     How manymicroliters are in 35 liters

                                                                                                                  

 

4.     How manygrams are in 2.2 kilograms?

                                                                                                                  

 

5.     How manymilligrams in 54,000 micrograms?

                                                                                                                  

 

6.     How manymillimeters are in 25 centimeters?

                                                                                                                  

 

7.     How manymilliliters are in 420 cubic centimeters (cc)?

                                                                                                                  

 


DATA ANALYSIS

 

     Thedata obtained from scientific research or laboratory exercises can beinterpreted when presented in a table, graph or chart. Tabular values areusually presented in tables as raw data or treated data.

 

Define the following terms used in theanalysis of data:

 

Average or mean

                                                                                                                  

 

Mode

                                                                                                                  

 

Range

                                                                                                                  

 

     Graphicalrepresentation of the data allows for a quick pictorial analysis of theinformation. A graph usually has two variables observed in the experiment withone plotted along the horizontal x-axis and the other variable along thevertical y-axis. The relationship between the variables of a graph can linearor curvilinear and show a positive or direct relationship, a negative orinverse relationship, a neutral relationship where the dependent variable isconstant or some other relationship. See Figure 1-4.

 

 

 

 

 

 

 


          A                    B                      C                     D                          E

 

Figure 1-4. Relationships between thevariables of a graph.

Graphs A, B and C are linear graphs witha graph A having a positive or direct relationship, graph B a negative orinverse relationship and graph C as being constant. Graph D shows a positivecurvilinear relationship and E a negative curvilinear relationship.

 

     Theindependent variable is changed, usually at regular intervals, in order toobserve its effect on the dependent variable. Most graphs plot the independentvariable on the abscissa (horizontal x-axis) and the dependent variable on theordinate (vertical y-axis). Graphs can be line, histograms or scatter. SeeFigures 1-5 and 1-6.

     Agraph is first constructed by differentiating between the dependent andindependent variables, and their axes. The spacing of the tabular data on theaxes is important. This is achieved by spreading the values out along the axesand by using the same distances for equivalent values. The first datum point isthen ready for plotting on the graph. The values for both variables are locatedon their respective axes. Each value is then moved either vertically orhorizontally until both of them intersect for the datum point. This process isthen continued for all values. Finally all the data points on the graph areconnected point to point with straight lines.

 

 

Table 1-4. Age and Weight in Boys.

 

Age (years)

Weight (kilograms)

Age (years)

Weight (kilograms)

0

3.4

10

32.6

1

10.1

11

35.2

2

12.6

12

38.3

3

14.6

13

42.2

4

16.5

14

48.8

5

18.9

15

54.5

6

21.9

16

58.8

7

24.5

17

61.8

8

27.3

18

63.1

9

29.9

 

 

 

     Whichfactor on Table 1-4 is the independent variable?

                                                                                                                  

 

     Whichaxis of a graph is the independent variable usually plotted on?

                                                                                                                  

 

 

 

     What is therelationship between age and body weight as seen in Figure 1-5 and
Figure 1-6.

                                                                                                                  

                                                                                                                  

 

     Whatare the two age ranges in Figures 1-5 and 1-6 that show a dramatic increase inbody weight?

                                                                                                                  

                                                                                                                  

Figure 1-5. Line Graph of the BodyWeight Data.

 

 

 

Figure 1-6. Bar Chart of the BodyWeight Data.

 

     Datacan also be visualized in a chart such as a bar chart or pie diagram. A piechart is a circular diagram that is divided into sections that represent acategory of data. The total area or all of the data is usually expressed as 100percent. See Figure 1.7.

 

 

Figure1-7. Age Distribution at Mesa College.

 

 

 

 

GRAPHING DATA

 

OXYGENAND HEMOGLOBIN

 

Table 1-5. Relationship Between OxygenLevels and Percent Saturation.

 

Partial Pressure (Concentration) of O2 (mmHg)

 

10

 

20

 

30

 

40

 

50

 

60

 

70

 

80

 

90

 

100

Percent O2 Saturation on Hemoglobin

 

14

 

35

 

60

 

75

 

84

 

89

 

92

 

95

 

96

 

97

 


     What is the independent variable on Table1-5?

                                                                                                                  

 

     What is thespecific variable from Table 1-5 is plotted on the vertical axis?

                                                                                                                  

 

     Label both axes correctly on Figure1-8 and plot the data from Table 1-5 on Figure 1-8. Draw a line connectingdatum point to datum point.

 

 

Figure 1-8. Hemoglobin Saturationverses Oxygen Levels.

 

 

     Describe therelationship between the partial pressure of oxygen gas to the percent ofoxygen saturation on hemoglobin.

                                                                                                                  

                                                                                                                  

                                                                                                                                     

                                                                                                                                     

                                                                                                                  

 


ROD CELLS

 

Table 1-6. Rod Cell Sensitivity toDifferent Wavelengths.

 

Color

violet

blue

green

yellow

orange

red

 

Wavelength (nanometers)

400

425

450

475

500

525

550

575

600

625

650

Rod Cell Sensitivity

20

30

60

90

100

85

40

15

5

0

0

 

     Plotthe data from Table 1-6 on Figure 1-9 and draw a line connecting the datapoints.


 

Color

                violet       blue            green            yellow         orange        red

 

Figure 1- 9. Rod Cell Sensitivityversus Wavelength.

 


 

     Whichwavelength(s) of light activate the rod cells the most?

                                                                                                                  

 

     Whichcolor of light are the rod cells the least sensitive to?

                                                                                                                  

 

     Describe how rod cell sensitivitychanges with wavelength in Figure 1-8?

                                                                                                                  

                                                                                                                  

                                                                                                                  

                                                                                                                  

                                                                                                                                                                                                                                                         

                                                                                                                  

 

 


Notes